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KMID : 0364819880260010006
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Korean Journal of Microbiology
1988 Volume.26 No. 1 p.6 ~ p.12
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Expression of Cellulomonas biazotea Cellobiase Gene in E. coli
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Abstract
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1
Cellobiase ( fl-glucosidase) is an enzyme of the cellulose system in cellalolytic microorPulsms.
The chromosomal DNA fragment which include cellobiase gene of Ceiulomonas biazotea was cloned in Eseherichla coil via plasmid pBR 322 vector. Restriction enzyme Sal I was used to obtain adequate size of fragments from C. blazoteam chromosomal DNA.
The transformant of E. colt HB101 with recombinant plasmid pBG 101 showed cellobiase activity, which is not ordinary in E. coli HB101.
The enzyme activity of the transformant was as of 20% lower than that of C. biazotea.
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